Rangaiah Shashidharamurthy, PhD
Assistant Professor of Pharmaceutical Sciences
PhD, Department of Biochemistry, University of Mysore, India, 2001
MS, Department of Biochemistry, University of Mysore, India, 1996
BS, University of Mysore, India, 1994
Dr. Shashidharamurthy's initial interest in teaching started during his training towards
obtaining a PhD degree. Upon entering the Ph.D program, the most influential of his
mentors were those who successfully achieved a career by combining teaching with research.
As a graduate student, Dr. Shashidharamurthy learned to appreciate the aspects of
teaching and research that an academic career could provide. At PCOM-SOP, he mentors
young scientists while teaching courses in Immunology, Biochemistry, Infectious Diseases,
Integrated Therapeutics and Toxicology to PharmD students. In addition to teaching,
he has also been involved in mentoring and supervising many work-study students, research
assistants and postdoctoral fellows.
One of the major focuses of Dr. Shashidharamurthy's research is to delineate the molecular
mechanism(s) lipocalin-2 (Lcn2) during various autoimmune/inflammatory disorders such
as arthritis and lupus. This may eventually lead to development of novel therapeutic
approaches. More recently his research has shown that Lcn2 plays a protective role
during the pathogenesis of immune-complex mediated arthritis (Arthritis Rheum. 2013;65:1064-73).
The preliminary data suggests that Lcn2 knockout mice are defective in the production
of anti-inflammatory cytokines and T reg expansion during arthritis condition. These
results demonstrate that Lcn2 is a host-protective factor against systemic autoimmune
disease. Apart from this project, our laboratory is also focused on: The individual
role of FcGamma receptor and complement proteins during the pathogenesis of immune-complex
(IC) mediated vasculitis (currently funded by American Heart Association), Attenuation
of IC-mediated inflammation by prostaglandin E2 receptor antagonists and Synergistic
effect of IC’s and TLR ligands during IC-mediated inflammatory disorders.
Early career Faculty Travel Award from American Association of Immunologist. 2013
American Association of Immunologist Trainee Awards. 2011 (two awards for two different
Scientist Development grant program Award from America Heart Association. 2011
Mula RV and Shashidharamurthy R. Multifaceted role of TNF-α during Rheumatoid arthritis. Adv Biosci Biotech. 2013;4:937-940.
Shashidharamurthy R*, Machiah D, Aitken JD, Putty K, Srinivasan G, Chassaing B, Parkos CA, Selvaraj P,
Matam VK. Differential role of lipocalin-2 during immune-complex mediated acute and
chronic inflammation.Arthritis Rheum. 2013;65:1064-73.
Ganesh T, Jiang J, Shashidharamurthy R, Dingledine R. Discovery and characterization of carbamothioylacrylamides as EP2
selective antagonists. ACS Med Chem Lett. 2013;4:616-621.
Bozeman EN, Cimino-Mathews A, Machiah DK, Patel JM, Krishnamoorthy A, Tien L, Shashidharamurthy R, Selvaraj P. Expression of membrane anchored cytokines and B7-1 alters tumor microenvironment
and induces protective antitumor immunity in a murine breast cancer model. Vaccine. 2013;31:2449-56.
Srinivasan G, Aitken JD, Zhang B, Carvalho FA, Chassaing B, Shashidharamurthy R, Borregaard N, Jones DP, Gewirtz AT, Vijay-Kumar M. Lipocalin 2 deficiency dysregulates
iron homeostasis and exacerbates endotoxin-induced sepsis. J Immunol. 2012;15:189:1911-9.
Bozeman EN, Srivatsan S, Mohammadi H, Shashidharamurthy R, Daniels D, Selvaraj P. Ukrain, a plant derived semi-synthetic compound, exerts antitumor
effects against murine and human breast cancer and induces protective immunity in
mice. Exp Oncol. 2012;34:340-7.
Shashidharamurthy R, Bozeman E, Selvaraj P. Administration of plasmid DNA encoding human FcγRs blocks the immune-complex mediated inflammation in vivo in reverse passive Arthus
reaction model. Gene Ther. 2012;19:877-85.
D'Souza B, Bhowmik T, Shashidharamurthy R, Oettinger C, Selvaraj P, D'Souza M.Oral microparticulate vaccine for melanoma using
M-cell targeting. J Drug Target. 2012 Feb;20(2):166-73
Shashidharamurthy R, Bozeman E, Patel J, Selvaraj P. Immunotherapeutic strategies for cancer treatment:
A novel protein transfer approach as an alternative for cancer vaccine development.
Med Res Rev. 2012 Nov;32:1197-219.
Bhowmik T, D’Souza B, Shashidharamurthy R, Oettinger C, Selvaraj P and D’Souza MJ. A novel microparticulate vaccine for melanoma
cancer using transdermal delivery. J. Microencap, 2011;28:294-300.
Goins C.L, Chappell C.P, Shashidaramurthy R, Selvaraj P and Jacob J. Immune complex mediated enhancement of secondary antibody
responses. J Immunol. 2010;184:6293-6298.
Bozeman E, Shashidharamurthy R, Paulos S.A, Palaniappan R, D’Souza M, Selvaraj P. Cancer vaccine development: Designing
tumor cells for greater immunogenicity. Frontiers In Bioscience, 2010; 15: 309-320.
Shashidharamurthy R, Ravichandran P, Amono A, Zhang F, Ezekwudo D, Amano A, Zhu C, Selvaraj P. Dynamics
of the interaction of human IgG subtype immune-complexes with cells expressing R and
H allelic forms of a low affinity Fc gamma receptor CD32A. J Immunol. 2009; 183: 8216-8224.
Bozeman E, Jennifer H, Shashidharamurthy R, Selvaraj P. Commentaries on role of PD1 molecules in bacterial and viral infections.
Immunotherapy. 2009; 1: 525-527.
Bumgarner GW, Shashidharamurthy R, Nagarajan S, D’Souza MJ, and Selvaraj P. Surface engineering of microparticles using
a novel protein transfer technique: Potential applications in vaccine adjuvant development
and targeted drug delivery. J. Control release. 2009;137:90-97.
Ezekwudo D, Shashidharamurthy R, Devineni D, Palaniappan R, Selvaraj P. Down regulation of anti-apoptotic protein
and induction of cell death in radioresistant human prostate adenocarcinoma cell line
(PC-3) by methyl jasmonate. Cancer Lett. 2008: 270:277-285.
Selvaraj P, Yerra A, Tien L and Shashidharamurthy R. Custom designing therapeutic cancer vaccines: Delivery of immunostimulatory molecule
adjuvants by protein transfer. Hum Vaccin. 2008; 4: 384 – 388.
Shashidharamurthy R, Hennigar RA, Fuchs S, Palaniswami P, Sherman M, Selvaraj P. Extravasations and emigration
of neutrophils to the inflammatory site depend on the interaction of immune-complex
with Fcγreceptors and can be effectively blocked by decoy Fcγ receptors. Blood. 2008; 111:894-904.
Shashidharamurthy R, Amano A, Ezekwudo D, Selvaraj P. Analysis of competitive interaction of H and R
allelic forms of CD32A with rabbit IgG immune-complex. Proc. Intl. Cong. Immunol. 2007, p513-522.
Wang YC, Sashidharamurthy R, Nagarajan S, Selvaraj P. B7-1-HSA (CD80-CD24), a recombinant hybrid costimulatory
molecule retains ligand binding and costimulatory functions. Immunol Lett. 2006; 105. 185-92.
Shashidharamurthy R, Koteiche HA, Dong J, McHaourab HS. Mechanism of chaperone function in small heat
shock proteins: dissociation of the HSP27 oligomer is required for recognition and
binding of destabilized T4 lysozyme. J Biol Chem. 2005; 280. 5281-9.
Stanic AK, Shashidharamurthy R, Bezbradica JS, Matsuki N, Yoshimura Y, Miyake S, Choi EY, Schell TD, Van Kaer L,
Tevethia SS, Roopenian DC, Yamamura T, Joyce S. Another view of T cell antigen recognition:
cooperative engagement of glycolipid antigens by Va14Ja18 natural T(iNKT) cell receptor.
J Immunol. 2003; 17. 4539-51.
Yadav R, Yoshimura Y, Boesteanu A, Christianson GJ, Ajayi WU, Shashidharamurthy R, Stanic AK, Roopenian DC, Joyce S. The H4b minor histocompatibility antigen is caused
by a combination of genetically determined and posttranslational modifications. J Immunol. 2003; 170. 5133-42.
Shashidharamurthy R, Bozeman EN, Aitken JD, Selvaraj P, Vijay-Kumar M. Regulatory T cell dysfunction
in lipocalin 2 knockout mice exacerbates serum-induced arthritis. American Association
of Immunologist, Honolulu, Hawaii, USA. May 3-7. 2013.
Shashidharamurthy R, Aitken JD, Srinivsan G, Selvaraj P, Vijay-Kumar M. Lipocalin-2, an innate immune
protein plays a key role in antibody-mediated inflammation. Keystone Sympoia, Santa
Fe, New Mexico, USA. January 19 - 24, 2012.
Aitken JD, Srinivsan G, Shashidhramurthy R, Parkos CA, Selvaraj P and Vijay-Kumar M. Lipocalin 2, an innate immune protein plays
a key role in immune-complex (IC) mediated inflammation. FASEB, San Diego, CA, USA.
April 21 - 25, 2012.
Huang C, Putty K, Wu Y, Zhu C, Selvaraj P, Shashidharamurthy R. Effect of tumicamycin treatment on the cell surface expression and function of low
affinity Fcgamma receptor CD16. PCOM, GA, USA. Annual Research Day. May 2, 2012.
Putty K, Bozeman E, Huang C, Wu Y, Selvaraj P, Vijay-Kumar M, Shashidharamurthy R. Lipocalin-2, an innate immune protein plays a key role in collagen antibody-mediated
arthritis. PCOM, GA, USA. Annual Research Day. May 2, 2012.
Shashidharamurthy R, Patel J, Bozeman E, Selvaraj P. Suppression of inflammation in collagen induced
arthritis by administration of recombinant Fcgamma receptors. The American Association
of Immunologist annual meeting, San Francisco, USA, May 13-17, 2011. (Oral presentation).
Shashidharamurthy R, Parkos CA, Selvaraj P, Vijay-Kumar M. Lipocalin-2 is involved in immune-complex
(IC) mediated inflammation. The American Association of Immunologist annual meeting,
San Francisco, USA, May 13-17, 2011. (Oral presentation).
Shashidharamurthy R, Bozeman E, Patel J, Kaur R, Meganathan J, Selvaraj P. Analysis of cross-species
IgG binding to human and mouse Fcgamma receptor (FcγR). The American Association of Immunologist annual meeting, Baltimore, USA, May 7-12,
Shashidharamurthy R, Panchanathan R, Fiske E.D, Tien L, Selvaraj P. Melanoma cells expressing membrane-anchored
IL-2 and IL-12 induce effective antitumor immunity in the presence or absence of B7-1
costimulation. The American Association of Immunologist annual meeting, Miami, USA,
FASEB Journal.22:1078.21. 2008
Shashidharamurthy R, A. Amano, P. Selvaraj. Dynamics of interaction of immune complex with cells expressing
H and R allelic forms of a low affinity Fc gamma receptor CD32A. 13th International
Congress of Immunology, Rio de Janeiro, Brezil, August 21-25, 2007. (Oral and poster
Shashidharamurthy R, Ravichandran P, Selvaraj P. Recombinant soluble human CD16A-Ig homodimer inhibits
the immune complex-induced inflammation mediated by FcγRs in vivo in a mouse Reversed
Passive Arthus reaction model. The American Association of Immunologist annual meeting,
Boston, USA, May 12-26, 2006.
Role: Principal Investigator
Award type: Scientist Development Grant Program
Funding source: American Heart Association
Title: Blocking of immune-complex mediated vasculitis using recombinant Fc receptors.
Role: Principal Investigator
Award type: Internal funding
Funding Agency: (Chief Scientific officer) CSO-funding
Title: Synergistic effect of immune-complex and TLR ligand during immune-complex mediated
Role: Principal Investigator
Award type: Internal funding
Funding Agency: CSO-funding for MS-Thesis
Title: Attenuation of antibody- mediated inflammation by prostaglandin E2 receptor
atagonists during autoimmune arthritis.
Volunteer & Leadership
Reviewer, Biochimie, 2004- Present
Reviewer, Comparative Biochemistry and Physiology, 2004- present
Reviewer, Planta Medica, 2006- present
Reviewer, Nutrition Journal, 2007- present
Reviewer, Current Medicinal Chemistry, 2008-present
Reviewer, Immunopharmacology And Immunotoxicology, 2008-present
Reviewer, Archives of Biochemistry and Biophysics, 2009-present
Reviewer, Bioorganic & Medicinal Chemistry Letters, 2010-present
Reviewer, European Journal of Cancer, 2013-Present
Reviewer, Clinical and Applied Thrombosis/Hemostasis, 2013-present