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Rangaiah Shashidharamurthy, PhD

Rangaiah Shashidharamurthy, PhD

Assistant Professor of Pharmaceutical Sciences

Contact
Ph: 678-407-7344
FX: 678-407-7347
E-mail: rangaiahsh@pcom.edu

Education

PhD, Department of Biochemistry, University of Mysore, India, 2001
MS, Department of Biochemistry, University of Mysore, India, 1996
BS, University of Mysore, India, 1994

Narrative

Dr. Shashidharamurthy's initial interest in teaching started during his training towards obtaining a PhD degree. Upon entering the Ph.D program, the most influential of his mentors were those who successfully achieved a career by combining teaching with research. As a graduate student, Dr. Shashidharamurthy learned to appreciate the aspects of teaching and research that an academic career could provide. At PCOM-SOP, he mentors young scientists while teaching courses in Immunology, Biochemistry, Infectious Diseases, Integrated Therapeutics and Toxicology to PharmD students. In addition to teaching, he has also been involved in mentoring and supervising many work-study students, research assistants and postdoctoral fellows.

One of the major focuses of Dr. Shashidharamurthy's research is to delineate the molecular mechanism(s) lipocalin-2 (Lcn2) during various autoimmune/inflammatory disorders such as arthritis and lupus. This may eventually lead to development of novel therapeutic approaches. More recently his research has shown that Lcn2 plays a protective role during the pathogenesis of immune-complex mediated arthritis (Arthritis Rheum. 2013;65:1064-73). The preliminary data suggests that Lcn2 knockout mice are defective in the production of anti-inflammatory cytokines and T reg expansion during arthritis condition. These results demonstrate that Lcn2 is a host-protective factor against systemic autoimmune disease. Apart from this project, our laboratory is also focused on: The individual role of FcGamma receptor and complement proteins during the pathogenesis of immune-complex (IC) mediated vasculitis (currently funded by American Heart Association), Attenuation of IC-mediated inflammation by prostaglandin E2 receptor antagonists and Synergistic effect of IC’s and TLR ligands during IC-mediated inflammatory disorders.

Honors/Awards

Early career Faculty Travel Award from American Association of Immunologist. 2013 American Association of Immunologist Trainee Awards. 2011 (two awards for two different presentations)

Scientist Development grant program Award from America Heart Association. 2011

Selected Publications

Mula RV and Shashidharamurthy R. Multifaceted role of TNF-α during Rheumatoid arthritis. Adv Biosci Biotech. 2013;4:937-940.

Shashidharamurthy R*, Machiah D, Aitken JD, Putty K, Srinivasan G, Chassaing B, Parkos CA, Selvaraj P, Matam VK. Differential role of lipocalin-2 during immune-complex mediated acute and chronic inflammation.Arthritis Rheum. 2013;65:1064-73.

Ganesh T, Jiang J, Shashidharamurthy R, Dingledine R. Discovery and characterization of carbamothioylacrylamides as EP2 selective antagonists. ACS Med Chem Lett. 2013;4:616-621.

Bozeman EN, Cimino-Mathews A, Machiah DK, Patel JM, Krishnamoorthy A, Tien L, Shashidharamurthy R, Selvaraj P. Expression of membrane anchored cytokines and B7-1 alters tumor microenvironment and induces protective antitumor immunity in a murine breast cancer model. Vaccine. 2013;31:2449-56.

Srinivasan G, Aitken JD, Zhang B, Carvalho FA, Chassaing B, Shashidharamurthy R, Borregaard N, Jones DP, Gewirtz AT, Vijay-Kumar M. Lipocalin 2 deficiency dysregulates iron homeostasis and exacerbates endotoxin-induced sepsis. J Immunol. 2012;15:189:1911-9.

Bozeman EN, Srivatsan S, Mohammadi H, Shashidharamurthy R, Daniels D, Selvaraj P. Ukrain, a plant derived semi-synthetic compound, exerts antitumor effects against murine and human breast cancer and induces protective immunity in mice. Exp Oncol. 2012;34:340-7.

Shashidharamurthy R, Bozeman E, Selvaraj P. Administration of plasmid DNA encoding human FcγRs blocks the immune-complex mediated inflammation in vivo in reverse passive Arthus reaction model. Gene Ther. 2012;19:877-85.

D'Souza B, Bhowmik T, Shashidharamurthy R, Oettinger C, Selvaraj P, D'Souza M.Oral microparticulate vaccine for melanoma using M-cell targeting. J Drug Target. 2012 Feb;20(2):166-73

Shashidharamurthy R, Bozeman E, Patel J, Selvaraj P. Immunotherapeutic strategies for cancer treatment: A novel protein transfer approach as an alternative for cancer vaccine development. Med Res Rev. 2012 Nov;32:1197-219.

Bhowmik T, D’Souza B, Shashidharamurthy R, Oettinger C, Selvaraj P and D’Souza MJ. A novel microparticulate vaccine for melanoma cancer using transdermal delivery. J. Microencap, 2011;28:294-300.

Goins C.L, Chappell C.P, Shashidaramurthy R, Selvaraj P and Jacob J. Immune complex mediated enhancement of secondary antibody responses. J Immunol. 2010;184:6293-6298.

Bozeman E, Shashidharamurthy R, Paulos S.A, Palaniappan R, D’Souza M, Selvaraj P. Cancer vaccine development: Designing tumor cells for greater immunogenicity. Frontiers In Bioscience, 2010; 15: 309-320.

Shashidharamurthy R, Ravichandran P, Amono A, Zhang F, Ezekwudo D, Amano A, Zhu C, Selvaraj P. Dynamics of the interaction of human IgG subtype immune-complexes with cells expressing R and H allelic forms of a low affinity Fc gamma receptor CD32A. J Immunol. 2009; 183: 8216-8224.

Bozeman E, Jennifer H, Shashidharamurthy R, Selvaraj P. Commentaries on role of PD1 molecules in bacterial and viral infections. Immunotherapy. 2009; 1: 525-527.

Bumgarner GW, Shashidharamurthy R, Nagarajan S, D’Souza MJ, and Selvaraj P. Surface engineering of microparticles using a novel protein transfer technique: Potential applications in vaccine adjuvant development and targeted drug delivery. J. Control release. 2009;137:90-97.

Ezekwudo D, Shashidharamurthy R, Devineni D, Palaniappan R, Selvaraj P. Down regulation of anti-apoptotic protein and induction of cell death in radioresistant human prostate adenocarcinoma cell line (PC-3) by methyl jasmonate. Cancer Lett. 2008: 270:277-285.

Selvaraj P, Yerra A, Tien L and Shashidharamurthy R. Custom designing therapeutic cancer vaccines: Delivery of immunostimulatory molecule adjuvants by protein transfer. Hum Vaccin. 2008; 4: 384 – 388.

Shashidharamurthy R, Hennigar RA, Fuchs S, Palaniswami P, Sherman M, Selvaraj P. Extravasations and emigration of neutrophils to the inflammatory site depend on the interaction of immune-complex with Fcγreceptors and can be effectively blocked by decoy Fcγ receptors. Blood. 2008; 111:894-904.

Shashidharamurthy R, Amano A, Ezekwudo D, Selvaraj P. Analysis of competitive interaction of H and R allelic forms of CD32A with rabbit IgG immune-complex. Proc. Intl. Cong. Immunol. 2007, p513-522.

Wang YC, Sashidharamurthy R, Nagarajan S, Selvaraj P. B7-1-HSA (CD80-CD24), a recombinant hybrid costimulatory molecule retains ligand binding and costimulatory functions. Immunol Lett. 2006; 105. 185-92.

Shashidharamurthy R, Koteiche HA, Dong J, McHaourab HS. Mechanism of chaperone function in small heat shock proteins: dissociation of the HSP27 oligomer is required for recognition and binding of destabilized T4 lysozyme. J Biol Chem. 2005; 280. 5281-9.

Stanic AK, Shashidharamurthy R, Bezbradica JS, Matsuki N, Yoshimura Y, Miyake S, Choi EY, Schell TD, Van Kaer L, Tevethia SS, Roopenian DC, Yamamura T, Joyce S. Another view of T cell antigen recognition: cooperative engagement of glycolipid antigens by Va14Ja18 natural T(iNKT) cell receptor. J Immunol. 2003; 17. 4539-51.

Yadav R, Yoshimura Y, Boesteanu A, Christianson GJ, Ajayi WU, Shashidharamurthy R, Stanic AK, Roopenian DC, Joyce S. The H4b minor histocompatibility antigen is caused by a combination of genetically determined and posttranslational modifications. J Immunol. 2003; 170. 5133-42.

Selected Presentations

Shashidharamurthy R, Bozeman EN, Aitken JD, Selvaraj P, Vijay-Kumar M. Regulatory T cell dysfunction in lipocalin 2 knockout mice exacerbates serum-induced arthritis. American Association of Immunologist, Honolulu, Hawaii, USA. May 3-7. 2013.

Shashidharamurthy R, Aitken JD, Srinivsan G, Selvaraj P, Vijay-Kumar M. Lipocalin-2, an innate immune protein plays a key role in antibody-mediated inflammation. Keystone Sympoia, Santa Fe, New Mexico, USA. January 19 - 24, 2012.

Aitken JD, Srinivsan G, Shashidhramurthy R, Parkos CA, Selvaraj P and Vijay-Kumar M. Lipocalin 2, an innate immune protein plays a key role in immune-complex (IC) mediated inflammation. FASEB, San Diego, CA, USA. April 21 - 25, 2012.

Huang C, Putty K, Wu Y, Zhu C, Selvaraj P, Shashidharamurthy R. Effect of tumicamycin treatment on the cell surface expression and function of low affinity Fcgamma receptor CD16. PCOM, GA, USA. Annual Research Day. May 2, 2012.

Putty K, Bozeman E, Huang C, Wu Y, Selvaraj P, Vijay-Kumar M, Shashidharamurthy R. Lipocalin-2, an innate immune protein plays a key role in collagen antibody-mediated arthritis. PCOM, GA, USA. Annual Research Day. May 2, 2012.

Shashidharamurthy R, Patel J, Bozeman E, Selvaraj P. Suppression of inflammation in collagen induced arthritis by administration of recombinant Fcgamma receptors. The American Association of Immunologist annual meeting, San Francisco, USA, May 13-17, 2011. (Oral presentation).

Shashidharamurthy R, Parkos CA, Selvaraj P, Vijay-Kumar M. Lipocalin-2 is involved in immune-complex (IC) mediated inflammation. The American Association of Immunologist annual meeting, San Francisco, USA, May 13-17, 2011. (Oral presentation).

Shashidharamurthy R, Bozeman E, Patel J, Kaur R, Meganathan J, Selvaraj P. Analysis of cross-species IgG binding to human and mouse Fcgamma receptor (FcγR). The American Association of Immunologist annual meeting, Baltimore, USA, May 7-12, 2010.

Shashidharamurthy R, Panchanathan R, Fiske E.D, Tien L, Selvaraj P. Melanoma cells expressing membrane-anchored IL-2 and IL-12 induce effective antitumor immunity in the presence or absence of B7-1 costimulation. The American Association of Immunologist annual meeting, Miami, USA, FASEB Journal.22:1078.21. 2008

Shashidharamurthy R, A. Amano, P. Selvaraj. Dynamics of interaction of immune complex with cells expressing H and R allelic forms of a low affinity Fc gamma receptor CD32A. 13th International Congress of Immunology, Rio de Janeiro, Brezil, August 21-25, 2007. (Oral and poster presentation).

Shashidharamurthy R, Ravichandran P, Selvaraj P. Recombinant soluble human CD16A-Ig homodimer inhibits the immune complex-induced inflammation mediated by FcγRs in vivo in a mouse Reversed Passive Arthus reaction model. The American Association of Immunologist annual meeting, Boston, USA, May 12-26, 2006.

Grants

Role: Principal Investigator
Award type: Scientist Development Grant Program
Funding source: American Heart Association
Title: Blocking of immune-complex mediated vasculitis using recombinant Fc receptors.
Duration: 2011-2014
Amount: $308,000.00

Role: Principal Investigator
Award type: Internal funding
Funding Agency: (Chief Scientific officer) CSO-funding
Title: Synergistic effect of immune-complex and TLR ligand during immune-complex mediated disorders. 
Duration: 12/1/2012-12/31/2013
Amount: $12,000.00

Role: Principal Investigator
Award type: Internal funding
Funding Agency: CSO-funding for MS-Thesis 
Title: Attenuation of antibody- mediated inflammation by prostaglandin E2 receptor atagonists during autoimmune arthritis. 
Duration: 08/1/2012-08/31/2014
Amount: $15,000.00

Volunteer & Leadership

Editorial Service

Reviewer, Biochimie, 2004- Present
Reviewer, Comparative Biochemistry and Physiology, 2004- present
Reviewer, Planta Medica, 2006- present
Reviewer, Nutrition Journal, 2007- present
Reviewer, Current Medicinal Chemistry, 2008-present
Reviewer, Immunopharmacology And Immunotoxicology, 2008-present
Reviewer, Archives of Biochemistry and Biophysics, 2009-present
Reviewer, Bioorganic & Medicinal Chemistry Letters, 2010-present
Reviewer, European Journal of Cancer, 2013-Present
Reviewer, Clinical and Applied Thrombosis/Hemostasis, 2013-present

Last Updated: 4/16/15