Denah M. Appelt, PhD
Professor of Neuroscience, Physiology & Pharmacology
Department of Bio-Medical Sciences
Post-Doctoral Fellowship, Hahnemann University
PhD, Drexel University College of Medicine
BS, Juniata College
Dr. Appelt is a professor of Neuroscience in the department of Bio-Medical Sciences.
Dr. Appelt has served as the director for Basic and Clinical Neuroscience course in
the DO curriculum and the director of the Neuroscience course in the Biomedical master
program. Dr. Appelt lectures on neuroanatomy and participates in neuroanatomy labs
in the DO SPOM course, Physician Assistance, and Psychology program. In addition,
Dr. Appelt is the director for the dual degree DO/PhD. program in Cellular and Molecular
Biology designed to develop physician-scientist.
Dr. Appelt has done extensive research in the field of aging and is recognized for
her work on Alzheimer’s disease. Dr. Appelt is the director of PCOM’s Biological Imaging
Facility and is investigating pathogen involvement as a trigger for developing Alzheimer’s
disease. She is particular interesting in understanding the effects Chlamydia pneumoniae and HSV exert on the central nervous system that result in the neuropathology observed
in Alzheimer’s disease. Her laboratory uses high resolution electron microscopy, confocal
microscopy and epifluorescent microscopy to study cellular structures.
American Society for Cell Biology-Glenn Foundation Award for Outstanding Research
in Aging (1996)
Professor of the Year 2008 – Philadelphia College of Osteopathic Medicine
Kappa Sigma Phi Award for Osteopathic education (2003)
Lindback Distinguished Teaching Award (2013)
Appelt, D.M., and Balin, B.J.: Analysis of Paired Helical Filaments Found in Alzheimer Disease
Using Freeze-Drying/Rotary Shadowing. J. Struct. Biol. 111: 85-95, (1993).
Appelt, D.M., and Kopen, G.C., Boyne, L.J. , and Balin, B.J.: Localization of Transglutaminase
in Hippocampal Neurons: Implications for Alzheimer's Disease. J. Histochem. & Cytochem.
44: 1421-1427, 1996.
Appelt, D.M., and Balin, B.J.: The Association of Tissue Transglutaminase with Human Recombinant
Tau Results in the Formation of Insoluble Filamentous Structures. Brain Res. 745:21-31,
Balin, B.J., Gerard, H.C., Arking, E.J., Appelt, D.M., Branigan, P.J., Abrams, J.T., Whittum-Hudson, J.A.,and Hudson, A.P.: Identification
and Localization of Chlamydia pneumoniae in the Alzheimer's Brain. Med. Micro. & Immunol. 187:23-42: 1998.
Arking, E.J., Appelt, D.M., Abrams, J.T., Kolbe, S.J.,Hudson, A.P., and Balin, B.J.: Ultrastructural variation
of Chlamydia pneumoniae in the Alzheimer's brain. Pathogenesis. 1(3):201-211, 1999.
Balin, B.J., Loewy, A.R. and Appelt, D.M.:Analysis of Transglutaminase-catalyzed Isopeptide Bonds in Paired Helical Filaments
and Neurofibrillary Tangles From Alzheimer’s Disease. In:Amyloid and other protein
deposition: methods and models. (Methods in Enzymology 309:172-186, 1999; Academic
Press, Orlando, Fl; edited by Ronald Wetzel, Ph.D.).
Balin, B.J. and Appelt, D.M.:Transglutaminase-catalyzed Formation of Alzheimer-like Insoluble Complexes From Recombinant
Tau. In:Alzheimer’s Disease: Methods and Protocols. (In Methods in Molecular Medicine,
pp 395-403, The Humana Press, Inc., Totowa, NJ; edited by Dr. Nigel M. Hooper, 2000).
Little, C.S., MacIntyre, A., Hammond, C., Venuti, E., Bromke, B.J., Balin, B.J., Appelt, D.M.: Ultrastructural Analysis of Beta-Amyloid production in Monocytes, Epithelial, and
Endothelial Cells Infected with Chlamydia pneumoniae Isolated from Alzheimer Brains.
EXPO:Microscopy and Microanalysis. Volume 6 Supplement1 (411) 2000.
Hudson, A.P., Gerard, H.C., Whittum-Hudson, J.A., Appelt, D.M., Balin, B.J.: Chlamydia pneumoniae, APOEgenotype, and Alzheimer’s Disease. In:Chlamydia
pneumoniae and Chronic Diseases, pp 121-136, 2000; Springer-Verlag, Berlin, Germany;
edited by Johanna L’Age-Stehr).
Balin, B.J., and Appelt,D.M.: “Role of Infection in Alzheimer’s disease”: JAOA , 101(12)1-5, 2001 MacIntyre, A.,
Hammond, C.J., Little, C.S., Appelt, D.M., Balin, BJ.: Chlamydia pneumoniae infection alters the junctional complex proteins of human brain microvascular endothelial
cells. FEMS Microbiol. Lett. 217(2) 167-72, 2003
MacIntyre, A., Abramov, R., Hammond, C.J., Little, C.S., Appelt, D.M., Balin, B.J.: Chlamydia pneumoniae infection of human brain endothelial cells and monocytes promotes the transmigration
of monocytes through an in vitro blood brain barrier. J Neurosci Res. Vol 71:(5) 2003
Balin, B.J., Hammond, C.J., Little, C.S., MacIntyre, A., and Appelt, D.M., Chlamydia Pneumoniae in the Pathogenesis of Alzheimer’s Disease, in Chlamydia pneumoniae Infection and Diseases, H. Friedman ed., Kluwer Academic Plenum, New York, NY, 2003.
Little, C. S., Hammond, C. J., MacIntyre, A., Balin, B. J., and Appelt, D. M.Chlamydia pneumoniae induces Alzheimer-like amyloid plaques in brains of BALB/c mice. Neurobiology of
Aging Neurobiology of Aging. 25(4):419-29, 2004 April.
Prozialeck W, Lamar, PC and Appelt, DM , Differential Expression of E-Cadherin, N-Cadherin and Beta-Catenin in Proximal
and Distal Segments of the Rat Nephron, Biomed Central:Phys4:10, 2004.
R.F. Itzhaki, M.A. Wozniak, D.M. Appelt, B.J. Balin Infiltration of the brain by pathogens causes Alzheimer's disease. Neurobiology
of Aging, 2004 May-JUN:25(5):619-27.
Balin, B.J., Hammond, C.J., Little, C.S, MacIntyre, A., Appelt, D.M.: Chlamydia pneumoniae in the Pathogenesis of Alzheimer’s disease. In:Chlamydia pneumoniae
Infection and Diseases. Kluwer Academic/Plenum Publishers, New York, New York; Edited
by Drs. M. Bendinelli, Y. Yamamoto, and H. Friedman, 2004, pp. 211-226.
Way, D.S., Albert, E.V., Hammond, C.J., Bell, M.G, Balin, B.J., & Appelt, D.M.,:Caspase Activity is Inhibited in Neuronal Cells Infected with Chlamydia pneumoniae:
Implications for Apoptosis in Alzheimer’s Disease, 10th ICAD & Related Disorders;Alzheimer’s
Disease New Advances, 2006; 295-299.
Hammond,C., Little, C.S,Longo, N, Procacci, C., Appelt,D., Balin, BJ: Antibiotic Alters Inflammationin the Mouse Brain During Persistent Chlamydia
pneumoniae Infection 10th ICAD & Related Disorders;Alzheimer’s Disease New Advances
Balin, B.J., Appelt, D.M., Little, C.S., Hammond, C.J., Whittum-Hudson, J.A., Hudson, A.P.: Chlamydophila (Chlamydia)
pneumoniae in Alzheimer’s disease. In: Microbial Infections of the Neurologic System.
Springer Publishers; Edited by Andreas Seyfang, H. Friedman, and M. Bendinelli, 2007.
Brian J. Balin, C. Scott Little, Christine J. Hammond, Denah M. Appelt, Judith A. Whittum-Hudson, Hervé C. Gérard, and Alan P. Hudson,. Chlamydophila pneumoniae
and the etiology of late-onset alzheimer's disease. J Alzheimers Dis. 2008; 13(4):371-380. PMID: 18487846
Appelt,D, Roupas,M, Way,D, Bell,MG, Albert,E Christine J. Hammond, and Brian J. Balin: Inhibition
of Apoptosis in Neuronal cells Infected with Chlamydophila (Chlamydia) pneumoniae (BMC Neurosci. 2008 Jan 24;9(1):13)
Brian J. Balin, C. Scott Little, Christine J. Hammond, Denah M. Appelt, Judith A. Whittum-Hudson, Hervé C. Gérard, and Alan P. Hudson, Chlamydophila pneumoniae and the etiology of late-onset Alzheimer’s disease, J. of Alzheimer Disease13 ,371-380
Galluzzi KE, Appelt DM, Balin BJ. Modern care for patients with Alzheimer disease: rationale for early intervention.
J Am Osteopath Assoc. 2010 Sep;110(9 Suppl 8):S37-42.PMID: 20926742
Balin, B.J., Hammond, C.J., Little, C.S., Appelt, D.M., Hingley, S.T.: Evidence for an Infectious Etiology in Alzheimer’s disease. In: Advanced
Understanding of Neurodegenerative Diseases, Raymond Chuen-Chung Chang (Ed.), ISBN:
978-953-307-529-7, Intech, Rijeka, Croatia, 2011
Thacker, J.D., Balin, B.J., Appelt, D.M., Sassi-Gaha, S., Purohit, M., Rest, R.F., Artlett, C.M.: Host-response to injury
and infection and development of a first-in-class, broad-spectrum, anti-infective
drug. Antimicrobial Agents and Chemo., epub Jan. 30, 2012.
1997-The Ultrastructural Analysis of Paired Helical Filaments found in Alzheimer’s
Disease: Pathology Dept., Univ. of Pennsylvania
1997-The Involvement of Transglutaminase and Isopeptidase in Alzheimer’s Disease:
1997-An Interplay of a Novel Isopeptidase and Transglutaminase in Deposits of Amyloid
and Tangles Found in Alzheimers Disease: Wyeth Ayerst
1998-Emerging Pathogens Associated With Alzheimer’s disease: Eli Lilly
2001-Pathogen Entry through the Blood Brain Barrier: Consequences of Chlamydia Pneumonia
Infection in Human Monocytes and Brain Endothelial Cells-AOA
2002- Induction of Alzheimer's Disease-like Pathology in the Brains of Young, Non-Transgenic,
BALB/c Mice Following Infection with Chlamydia pneumoniae: A Model for Late Onset/Sporadic
Alzheimer's Disease: Society for Neurosciences
2003-Chlamydia pneumoniae Infection: A Trigger for Alzheimer’s disease: Lankenau Institute
for Medical Research
2005-Neuropathological entities of Alzheimer's Disease are triggered following infection
of neuronal cells with Chlamydophila (Chlamydia) pneumoniae" Drexel University Medical
2008- Think Tank on Dementia: Eastern Pennsylvania Geriatrics Society
2008- Current Hypotheses of Alzheimer’s Disease: Should We Be Looking Outside The
2009-The Pathogen Hypothesis in Alzheimer’s Disease: MidWestern Osteopathic School
Volunteer & Leadership
Presidential Diversity Council 2013-present
Research committee, 2013-present
D.O. Admissions Committee, 1999-present
Faculty senate, chair, 2014-2015
Middle States accreditation self-study committee, section chair, 2012-2014
Faculty search committee, 2012-2013
Honors and Awards committee, 2010-2013
Institutional and Environmental Health and Safety committee, vice chair, 2009-2013
Chief Science Officer search committee, 2011-2012
Faculty Representative to Executive Faculty, 2009-2010
Appointment, Promotion and Tenure, 2003-2009
Faculty Senate, Secretary, 2007-2008
Challenging Views of Alzheimer’s Disease, PCOM Symposium Organizer, 2004
CCDA (7/1/2013 -6/30/2014) Co-PI: Bactericidal Implant Surfaces
Rose and Adolf Levis Foundation (1/01/2003-present) Co-PI; Chlamydia pneumoniae’s role in the pathogenesis of sporadic Alzheimer’s disease
CCDA (7/1/2012 -6/30/2013) Co-PI; Infection with Chlamydia Pneumoniae alters calcium-associated gene regulations and processes in neuronal cells and monocytes:
Implications for Alzheimer’s disease
CCDA (7/1/2012 -6/30/2013) Co-PI; Detection of Chlamydia pneumoniae in blood samples: A diagnostic screen for Alzheimer’s disease
CCDA (9/1/2011 -6/30/2012) PI; Analysis by Real-Time PCR of Autophagy and Apoptosis
in Neuronal Cells Infected with Chlamydia Pneumoniae
Health Research Formula Fund :(1/01/ 2009- 12/31/2013) PI; Dissecting the apoptosis
pathway affected by C. pneumoniae in Alzheimer's disease
CCDA (9/1/2008 -6/30/2009) PI; Chlamydophila (Chlamydia) pneumoniae (Cpn) promotes Aβ 1-42 amyloid processing in Neuronal Cells: A Pathogenic Trigger
for Alzheimer’s disease
CCDA (10/1/2007 -9/30/2008) PI; Effects on the apoptotic markers following a Chlamydial
infection in neurons and astrocytes: Implications for Alzheimer’s disease
Alzheimer Association 7/01/04 to 6/30/07) Co-investigator; Chlamydia pneumoniae infection of Mice triggers Alzheimer-like pathology
NIH R01ES006478-09A2 (7/2003-2008) Collaborator; Mechanisms of Cadmium Toxicity in
Epithelial Cells NIH/NIAID - R01- AI44055-01A1 (06/01/99 - 05/31/04). Co-investigator;
Chlamydia pneumoniae: a pathogen in Alzheimer’s disease?
NIH/R15: (5/01/01-06/30/03) Co-investigator; Immunosenescence and Chlamydia Pneumoniae”
Foundation for Research into the Diseases of Aging (6/01/99-7/01/2003) Co-investigator;
Investigation of Chlamydia pneumoniae in Diseases of the Aging
National Foundation for Infectious Diseases(6/01/99-7/01/2000)PI; Pathologic manifestations
induced by Chlamydia Pneumonia infected glial cells in AD
- Alzheimer’s disease
- Chlamydia pneumoniae